Assistant Technical Specialist - Department of Immunology and Immunotherapy - 107995 - Grade 6

This role is for a Research Assistant/Technician in Immunology and Immunotherapy focused on induced regulatory T cell (Treg) biology in autoimmune liver diseases. Key responsibilities include performing specialized techniques like flow cytometry, cell culture, and molecular analysis, while collaborating with researchers and ensuring laboratory compliance. Applicants need substantial practical experience in relevant techniques, knowledge of the Human Tissue Act and H&S policies, and the ability to work both independently and as part of a team.

• The role holder will plan and perform a variety of specialist techniques including but not exclusively:
• Isolation and enrichment of cell subsets from whole blood, and liver and the subsequent phenotypic and functional characterisation of the cells
• Culture and phenotype of cells
• T cell receptor and T cell receptor analysis with Citeseq
• Multi-colour flow cytometry and cell sorting including analysis of the data
• DNA and RNA extraction and TSDR analysis
• PCR techniques including real time PCR and endpoint PCR
• Analysis of TSDR epigenetic sequencing data
• The role will include, but is not limited to, the following duties:
• Sample administration to ensure correct storage, traceability and process timelines including communication with clients/customers, help with organisation of sample transfer and initiation of administrative processes for material transfer agreements as and where required and liaison with the Human Biomaterial Resource Centre.
• Biological sample preparation and use of the facility
• Identification and diagnosing instrument problems and affecting solutions
• Keeping accurate records of all analyses, evaluation and interpretation of results
• Analysing results and make recommendations to customers/collaborators.
• Modification and development of new methods as appropriate
• Collate and present data to service users including group leaders and their research groups, external collaborators and/or Industry clients, for example at seminars and as posters, including drafting academic publications or parts thereof.
• Collate and present data to service users including group leaders and their research groups, external collaborators and/or Industry clients,
• for example at seminars and as posters, including drafting academic publications or parts thereof.
• Maintenance and quality control of the facility ensuring that GCLP/GCP standards are achieved for assays where appropriate. Including day to day maintenance of relevant records
• Test and implement new approaches to ensure effectiveness and efficiency of the facility You will be responsible for assisting in managing the experimental laboratories.

• Use of AI in applications: We want to understand your genuine interest in the role and for the written elements of your application to accurately reflect your own communication style.
• Applications that rely too heavily on AI tools can appear generic and lack the detail we need to assess your skills and experience.
• Such applications will unlikely be progressed to interview.
• Positive service attitude and shows courtesy in dealing with others.
• Understands the importance of equality and diversity in the workplace
• Ability to identify and respond to equality and diversity issues in line with relevant policies and procedures
• Ability to work under minimal supervision but function as part of a team.
• Proficiency in commonly used software packages.
• In-depth practical knowledge of relevant technical and scientific methods.
• Knowledge of current H&S policies and ability to uphold same.
• Ability to organise and manage own work load and demonstrate flexibility in terms of work practices/requirements.
• Further particulars can be found here
• Further particulars can be found
• GCSE level 2 (A*- C) or equivalent level 2 qualification in mathematics and English.
• S ubstantial practical and technical experience of a wide range of techniques as stated above.
• ubstantial practical and technical experience of a wide range of techniques as stated above.
• Extensive relevant practical and technical workplace experience, professional qualification or degree in an appropriate subject.
• Extensive experience of similar laboratory-based work, together with familiarity with a wide range of laboratory equipment including the ability to work to GCLP standard.
• Knowledge of current legislation relevant to the role including the Human Tissue Act.
• Informal enquiries to Ye Oo, email: y.h.oo@bham.ac.uk